What is Akt antibody?

What is Akt antibody?

Akt Antibody detects endogenous levels of total Akt1, Akt2 and Akt3 proteins. The antibody does not cross-react with related kinases.

What is phospho AKT?

The serine/threonine protein kinase AKT is a key player in the regulation of cell survival. AKT functions to promote cell survival by inhibiting apoptosis through its ability to phosphorylate and inactivate several targets including Bad, caspase-9 and Forkhead transcription factor.

What is Thr308?

Abbreviations: Ser473=serine 473; Thr308=threonine 308.

Is PKB the same as Akt?

Protein kinase B (PKB), also known as Akt, is the collective name of a set of three serine/threonine-specific protein kinases that play key roles in multiple cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription, and cell migration.

How is Akt activated?

Akt activation is governed by a dual regulatory mechanism in which it is first recruited to the cellular plasma membrane by PIP3 through a direct interaction with the PH domain of Akt. Activated form of Akt can modulate the functions of many substrates responsible for cell-cycle progression.

What Happens When Akt is activated?

Activated Akt mediates downstream responses, including cell survival, growth, proliferation, cell migration and angiogenesis, by phosphorylating a range of intracellular proteins. The pathway is present in all cells of higher eukaryotes and is highly conserved.

Is Akt a tumor suppressor?

Several of these proteins (AKT, eIF4E, and both the p110α catalytic and p85α regulatory subunits of PI3K) can behave as oncoproteins when activated or overexpressed, while others (PTEN, FOXO, LKB1, TSC2/TSC1, NF1, and VHL) are tumor suppressors.

What is Ser473?

Phosphorylation of serine 473 (Ser473) residue is a target of the mTOR complex 2 (mTORC2) [17] and DNA-dependent protein kinase (DNA-PK) [18]. Maximal AKT activity is dependent on the phosphorylation status of both Thr308 and Ser473 residues [15].

Why is it called Akt?

The name Akt stands for Ak strain transforming. The origins of the Akt name date back to 1928, where J. Furth performed experimental studies on mice that developed spontaneous thymic lymphomas. Mice from three different stocks were studied, and the stocks were designated A, R, and S.

What kind of protein is Akt?

PKB is also known as Akt. Akt is a serine/threonine-specific protein kinase that plays a critical role in controlling the balance between survival and death pathways in cells. The Akt kinase family comprises three highly homologous isoforms: Akt1 (PKBα), Akt2 (PKBβ), and Akt3 (PKBγ) [4,14].

How is Akt inactivated?

The low levels of Src-p60 may also contribute to direct attenuation of PDK-1 activity. These effects will result in inactivation of Akt by loss of phosphorylation at Thr-308 and Ser-473. The net output during apoptosis is inactivation of FKHR signaling and inhibition of the survival pathway.

How does AKT stimulate cell growth?

Akt mediates cell survival by directly inhibiting proapoptotic proteins like Bcl-2-associated death promoter and caspase-9. Endothelial nitric oxide synthase (eNOS) activated by Akt generates nitric oxide, resulting in an increase in vascular permeability and cellular migration.

What is the sensitivity of phospho AKT antibody?

Specificity / Sensitivity. Phospho-Akt (Ser473) Antibody detects endogenous levels of Akt1 only when phosphorylated at Ser473. This antibody also recognizes Akt2 and Akt3 when phosphorylated at the corresponding residues.

What is the target antigen of AKT1?

The target antigen refers to the phosphorylated version of AKT, also known as AKT1 or AKT serine/threonine kinase 1. AKT is activated by phosphorylation at multiple sites, including Thr 308 and Ser 473.

How do you test for Akt in SDS?

Heat the sample to 95–100°C for 2–5 min and microcentrifuge for 1 min at 14,000 x g. Load the sample (15–30 µl) on SDS-PAGE (4–20%). Phospho-Akt (Thr308) Antibody detects endogenous levels of Akt only when phosphorylated at Thr308.

How is the phospho-specificity of the Jurkat antibody confirmed?

The phospho-specificity of the antibody was confirmed by treating the membrane with calf intestinal alkaline phosphatase (CIP) after Western transfer. Immunoprecipitation of phospho-Akt (Thr308) from Jurkat cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Phospho-Akt (Thr308) (D25E6) XP ® Rabbit mAb (lane 3).