What color do you expect Dcpip to be of photosynthesis is working as expected at the end of 30 minutes?

What color do you expect Dcpip to be of photosynthesis is working as expected at the end of 30 minutes?

blue
In this investigation, DCPIP (2,6-dichlorophenol-indophenol), a blue dye, acts as an electron acceptor and becomes colourless when reduced, allowing any reducing agent produced by the chloroplasts to be detected….Using a micro-centrifuge.

What wavelengths initiate photosynthesis?

Plant pigment molecules absorb only light in the wavelength range of 700 nm to 400 nm; this range is referred to as photosynthetically-active radiation. Violet and blue have the shortest wavelengths and the most energy, whereas red has the longest wavelengths and carries the least amount of energy.

How can DPIP be used in a photosynthesis experiment?

In this experiment, a blue dye (2,6-dichlorophenol-indophenol, or DPIP) will be used to replace NADPH in the light reactions. Since DPIP replaces NADPH in the light reactions, it will turn from blue to colorless when reduced during photosynthesis. This will allow you to monitor the rate of photosynthesis.

Why does the reduction of the dye Dcpip work best using the chloroplasts whose outer envelopes have been damaged during preparation?

The reduction of the dye DCPIP works best using the chloroplasts whose outer envelopes have been damaged during preparation because it means that the DCPIP can access the thylakoid membranes directly, without having to pass through the outer membranes, to accept electrons directly from the electron transport chain.

Why was 605 nm used when the absorbance of the other mixtures was obtained?

Answer 2: “You set the wavelength at 605 nm because that is the wavelength for which DPIP has its greatest absorbance (darkest color).

At what wavelength does each photosystem operate best at?

The reaction centers of the photosynthetic photosystems are pigments termed P-680 and P-700 . The reaction center of photosystem II which best absorbs light at a 680 nm wavelength. P-680 is a chlorophyll a molecule.

Why only certain wavelengths are used in photosynthesis?

Different kinds of pigments exist, and each absorbs only certain wavelengths (colors) of visible light. Pigments reflect the color of the wavelengths that they cannot absorb. All photosynthetic organisms contain a pigment called chlorophyll a, which humans see as the common green color associated with plants.

Which electromagnetic wave has the highest frequency?

Gamma rays
Gamma rays have the highest energies, the shortest wavelengths, and the highest frequencies. Radio waves, on the other hand, have the lowest energies, longest wavelengths, and lowest frequencies of any type of EM radiation.

Does DPIP reduce or oxidize?

DPIP gains electrons, thus it was reduced. This replaces NADP in the light reaction since DPIP has a higher affinity for electrons than does NADP. DPIP Lab-If you do not see a reaction in the positive control, what might explain this?

What is the purpose of using DPIP in this experiment?

The function of the DPIP in this experiment is to act as the electron acceptor, replacing the usual NADP found in plants. When the light shines on the active chloroplasts, the electrons are excited, which causes them to jump to a higher energy level thus reducing the DPIP.

What is responsible for Hill reaction in photosynthesis?

The Hill Reaction depends on electrons released during the light dependent stage of photosynthesis being picked up by the blue electron acceptor DCPIP. The reaction can only occur if the thylakoid membranes are illuminated as the light dependent stage stops in the dark (tube B in the procedure).

Why is the Hill reaction important?

The evolution of oxygen during the light-dependent steps in photosynthesis (Hill reaction) was proposed and proven by British biochemist Robin Hill. He demonstrated that isolated chloroplasts would make oxygen (O2) but not fix carbon dioxide (CO2).

Does the colour change when the DCPIP is exposed to light?

Tube 1 (leaf extract + DCPIP) colour changes until it is the same colour as tube 4 (leaf extract + distilled water). Tube 2 (isolation medium + DCPIP) no colour change. This shows that the DCPIP does not decolourise when exposed to light. Tube 3 (leaf extract + DCPIP in the dark) no colour change.

Why is the absorbance of DCPIP measured at 621 nm?

The absorbances readings recorded measured the amount of DCPIP oxidized in the cells. As photosynthesis occurred, electrons were donated to the DCPIP oxidized, forming DCPIPH 2 reduced. DCPIP oxidized is able to absorb light from the spectrophotometer at 621 nm, hence that it why it was calibrated at 621 nm.

How to measure the amount of DCPIP in Spinach chloroplast cells?

Using a spectrophotometer, the amount of DCPIP present was able to be determined and tests using variable intensities of light were performed on spinach chloroplast cells. First, a room was insulated from light and the lights were turned off. A green light was used in order to see.

How does the intensity of light affect the rate of photosynthesis?

The rate at which their absorbances went down increased as their distance from the light source decreased. The intensity of light directly affects the rate of photosynthesis (Bidwell, 1979). Graphs show that the higher the intensity, the higher rate of photosynthesis.