What are Cre driver mice?
What are Cre driver mice?
Cre-driver lines: A special type of transgenic mouse As different neurons have different cellular machinery to activate and express certain genes, Cre-recombinase was targeted to specific neuronal (or other brain cell types) with this in mind.
What are Cre lines?
The Cre-lox system is used as a genetic tool to control site specific recombination events in genomic DNA. The Cre protein is a site-specific DNA recombinase that can catalyse the recombination of DNA between specific sites in a DNA molecule.
What is Sox2 Cre?
Also Known As:Sox2Cre These Sox2Cre transgenic mice express Cre recombinase under the control of the mouse Sox2 (SRY-box containing gene 2) promoter, and may be useful for generating epiblast-derived specific conditional mutations.
Where is Cre in a neuron?
Cre is expressed in layer 6 corticothalamic neurons in an ntsr1 BAC-Cre line (B, Ntsr1). Clearly labeled are the projection axons of these neurons, which terminate in the dorsal thalamic nuclei.
What is nestin Cre?
This strain is commonly known as nestin-Cre. Nestin promotor driven Cre recombinase is expressed in the central and peripheral nervous system, including neuronal and glial cell precursors. Hemizygous nestin-cre mice exhibit mild hypopituitarism, growth retardation, impaired fear response, and a metabolic phenotype.
What are Cre rats?
Mouse models that provide this level of control are called conditional mouse models, of which one of the most effective is the Cre/Loxp mouse. Cre mice express Cre recombinase under the control of a tissue specific gene promoter, i.e. Cre mouse lines provide the tissue specificity of gene editing.
How does Flox CRE work?
How does it work? As alluded to above, the Cre recombinase catalyzes the site specific recombination event between two loxP sites, which can be located either on the same or on separate pieces of DNA. Both 13bp repeat sequences on a single loxP site are recognized and bound by a Cre protein, forming a dimer.
What is CP Cre?
CP-CRE, which stands for Carbapenemase Producing Carbapenem-resistant Enterobacteriaceae, are CRE that are capable of breaking down Carbapenems. CP-CRE are an important emerging threat to public health. Common Enterobacteriaceae include Klebsiella species and Escherichia coli (E. coli).
What is a CRE promotor?
Cre-loxP system is a widely used powerful technology for mammalian gene editing. First, Cre-driver strain is generated in which Cre recombinase is expressed by a promoter that specifically targets the cell or tissue of interest. Second, loxP flanked (floxed) DNA containing mouse strain is needed to be generated.
What is the prognosis of hemizygous mice with camk2a-creert2?
Mice hemizygous for the Camk2a-CreERT2 transgene are viable and fertile, with expression of CreER T2 fusion protein (CreERT2 fusion protein) directed to neural populations by the mouse calcium/calmodulin-dependent protein kinase II alpha promoter region.
What does CAMK2A stand for?
CaMKIIα-Cre line T29-1 transgenic mice have the mouse calcium/calmodulin-dependent protein kinase II alpha (Camk2a) promoter driving Cre recombinase expression in the forebrain, including the CA1 pyramidal cell layer of the hippocampus.
Do TG (camk2a-cre)t29-1stl mice show Cre recombinase expression in spermatocytes?
Importantly, Tg (Camk2a-cre)T29-1Stl mice exhibited evidence of Cre recombinase expression in several structures of the male germline, including spermatocytes.
Where is CAMK2A active in the brain?
The promoter is active in brain regions including the dentate gyrus, CA1/CA3 regions of the hippocampus, cortex, olfactory bulb and amygdala, and more weakly active in striatum, thalamus and hypothalamus. ( J:71980 ) Camk2a Summary of all recombinase alleles driven by Camk2a .