How do you prepare B5 media?

How do you prepare B5 media?

for 1 L product: Dissolve one bag (3.3 g; for 1 L preparation) of this product in one liter of distilled water, add growth regulators and vitamins as needed, and sterilize by autoclave at 120 °C for 15 minutes.

What is B5 medium?

Gamborg B5 medium is a nutrient blend of inorganic salts, vitamins and carbohydrate. Vitamins like thiamine, inositol, pyridoxine, nicotinic acid act as enzymatic cofactors in the universal pathways including glycolysis and TCA cycle along with primary and secondary metabolism in the plants.

How do you prepare woody plant media?

Reconstitute medium by adding required quantity of powder in two-third of total volume with constant, gentle stirring till the medium gets completely dissolved. Add heat stable supplements prior to autoclaving. Make up the final volume with distilled water. Adjust the pH of the medium to 5.75 ± 0.5 using 1N NaOH/ HCl.

What is Nitsch media?

Nitsch medium has been specially formulated for the in vitro culture of plant cell, tissue and organ culture. Ammonium nitrate and potassium nitrate serves as the sources of nitrate. Glycine serves as the source of amino acid. Sucrose serves as the carbohydrate source.

What are the ingredients of MS medium?


  • Ammonium nitrate (NH4NO3) 1650 mg/l.
  • Calcium chloride (CaCl2 · 2H2O) 440 mg/l.
  • Magnesium sulfate (MgSO4 · 7H2O) 370 mg/l.
  • Monopotassium phosphate (KH2PO4) 170 mg/l.
  • Potassium nitrate (KNO3) 1900 mg/l.

What is MS medium composition?

Murashige and Skoog Medium (MS) provides all the essential macroelements and microelements. Potassium dihydrogen phosphate serves as a source of phosphate. Microelements like Boron, Manganese, Molybdenum, Copper and Zinc play vital role in plant metabolism. Boron plays a key role in carbohydrate metabolism.

Which is the most commonly used medium in PTC?

MS medium is widely used in PTC because of its having balanced nutrients and most of the crops are grown well.

What are the ingredients for tissue culture?

The essential elements in plant cell or tissue culture media include, besides C, H and O, macroelements: nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg) and sulphur (S) for satisfactory growth and morphogenesis.

Why WPM is used for woody plants?

Woody Plant Medium (WPM) provides all the essential macroelements and microelements. Potassium dihydrogen phosphate serves as a source of phosphate while calcium nitrate provides nitrogen to the plant. Microelements like Boron, Manganese, Iron, Molybdenum and Zinc play a vital role in the plant metabolism.

What are woody plant species?

Woody plants are trees and shrubs whose shoots are durable and survive over a period of years. They are further classified into deciduous and evergreen plants.