Which technique can be used for identification of transgenic mice?

Which technique can be used for identification of transgenic mice?

Identification of transgenic mice by direct PCR analysis of lysates of epithelial cells obtained from the inner surface of the rectum.

What are TCR transgenic mice?

T cell receptor (TCR) Tg mice are extremely useful in studying T cell-mediated immune responses because the majority of T cells in these mice express specific TCRs for partner antigens.

How are the transgenic mice used in cancer research?

These mice provide valuable clues about the biological function of a normal gene. In translational cancer research, this represents a powerful tool in assessing the potential validity of targeted therapy because the targets can be precisely inactivated in the setting of a developing or developed tumor.

How are transgenic mice produced?

Typically, transgenic mice are generated by microinjecting the transgenic construct into a fertilized egg (oocyte or zygote). Another method to generate transgenic mice is to transfect a transgenic construct into mouse embryonic stem (ES) cells and then inject these cells into mouse blastocysts.

Why mice are used as transgenic animals?

Such work laid the basis for the creation of transgenic mice genetically modified to inherit particular forms of cancer. These mice were generated as a laboratory tool to better understand the onset and progression of cancer. The advantage of such mice is that they provide a model which closely mimics the human body.

What is meant by transgenic mice?

(trans-JEH-nik…) Mice that have had DNA from another source put into their DNA. The foreign DNA is put into the nucleus of a fertilized mouse egg. The new DNA becomes part of every cell and tissue of the mouse. These mice are used in the laboratory to study diseases.

What does the term transgenic refer to?

​Transgenic Transgenic means that one or more DNA sequences from another species have been introduced by artificial means. Animals usually are made transgenic by having a small sequence of foreign DNA injected into a fertilized egg or developing embryo.

What is OT II?

B6. Strain #004194 | Common Name: OT-II. These OT-II transgenic mice express the mouse alpha-chain and beta-chain T cell receptor that pairs with the CD4 co-receptor and is specific for chicken ovalbumin 323-339 peptide in the context of I-Ab (CD4 co-receptor interaction with MHC class II).

How are the transgenic mice used in cancer research Shaalaa?

Transgenic mice that have been modified using a particular oncogene (cancer-causing gene) and thus developed a certain type of cancer, are useful to answer questions concerning the relationship between oncogenes and cancer development.

Why are transgenic mice used?

Genetically modified mice are used extensively in research as models of human disease. Mice are a useful model for genetic manipulation and research, as their tissues and organs are similar to that of a human and they carry virtually all the same genes that operate in humans.

How are transgenic mice produced by retroviral vector method?

Microinjection of retrovirus into immature seminiferous tubules resulted in the direct transduction of spermatogonial stem cells in situ, and the animals produced transgenic offspring after mating with females. Transgenic mice were produced in C57BL/6, BALB/C, A, and C3H backgrounds, with an average efficiency of 2.8%.

Which is the first method used to produce the transgenic mice?

The first method involves DNA delivery by retroviral infection of mouse embryos at different developing stages.