What is acetylated BSA?

What is acetylated BSA?

Bovine Serum Albumin (BSA) Acetylated. Acetylated BSA is used as a carrier protein and an enzyme stabilizer in which the absence of contaminants such as nucleases and proteases is vital. The acetylation process inactivates any trace of active nuclease activity.

Can BSA inhibit PCR?

BSA is widely used as a PCR inhibition alleviating agent in microfluidic devices in both static11–15 and dynamic passivation modes. Also, there are many reports, where combinations of static and dynamic surface passivation using BSA have been applied to PCR micro-devices.

What is BSA in PCR?

BSA increases PCR yields from low purity templates. It also prevents adhesion of enzymes to the reaction tubes and tip surfaces.

How does BSA help in PCR?

BSA significantly enhances PCR amplification yield when used in combination with organic solvents, DMSO or formamide. When added to the reaction buffer, promoting effects of BSA were seen in the first cycles of the PCR, regardless of the size of the DNA to amplify.

How do you use BSA in PCR?

BSA (bovine serum albumin) is particularly useful to enhance the efficiency or specificity of PCR. The concentration depends on the template, but as a general rule, 0.01 µg/µl to 0.5 µg/ µl BSA (final concentration) can be used.

What is BSA in cell culture?

Bovine Serum Albumin (BSA) is commonly used in cell culture protocols, particularly where protein supplementation is necessary and the other serum components are unwanted. Binds water, salts, fatty acids, vitamins and hormones and carries these bound components between tissues and cells.

How do you make a BSA PCR solution?

I dissolved BSA in distilled water to make a series of standard solution for total protein assay. For a 10% (100 mg/mL) stock solution of BSA, dissolve 1 g powdered Fraction V or molecular biology grade BSA in 10 mL of distilled H2O; to avoid clumping, dissolve by layering the powder on the surface of the liquid.

What does Betaine do in PCR?

Betaine is the most common PCR additive used to enhance amplification of GC rich sequences because of its ability to dissolve secondary structure that blocks polymerase action.

What are PCR reagents?

Standard PCR reagents include a set of appropriate primers for the desired target gene or DNA segment to be amplified, DNA polymerase, a buffer for the specific DNA polymerase, deoxynucleotides (dNTPs), DNA template, and sterile water.

Why is BSA used as standard?

BSA is used because of its ability to increase signal in assays, its lack of effect in many biochemical reactions, and its low cost, since large quantities of it can be readily purified from bovine blood, a byproduct of the cattle industry.

What is BSA in biology?

Bovine Serum Albumin (BSA) is supplied with some products to prevent adhesion of the enzyme to reaction tubes and pipette surfaces. BSA also stabilizes some proteins during incubation.

What is Ambion® acetylated BSA?

Ambion® Acetylated BSA is used as a carrier protein and as an enzyme stabilizer in which the absence of contaminants such as nucleases and proteases is essential.

What is the concentration of acetylated BSA in PCR?

Six tubes of 25 mg are provided, at a concentration of 20 mg/mL. The acetylation process inactivates any trace nuclease activity that might be present. While the acetylation process does change BSA’s binding characteristics and makes it inhibitory in PCR reactions, it is routinely added to restriction digests.

What is bovine serum albumin (BSA)?

Bovine serum albumin (BSA) is widely used as an additive in polymerase chain reaction (PCR)-based microfluidic devices to passivate reactors and alleviate nucleic-acid amplification. BSA is available commercially in two types: either acetylated or non-acetylated. A survey of literature indicates tha …

What types of BSA are used in PCR-based microfluidic devices?

BSA is available commercially in two types: either acetylated or non-acetylated. A survey of literature indicates that both types of BSA are used in PCR-based microfluidic devices.