How do you make rifampicin stock solution?
How do you make rifampicin stock solution?
- Prepare a ml rifampicin stock solution ( mg ml-1) by adding. mg rifampicin.
- Dissolve in ml methanol (100%)
- Make aliquots and store at -20°C.
How do you dissolve rifampin?
Rifampicin is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF), which should be purged with an inert gas. The solubility of rifampicin in these solvents is approximately 0.12, 3.3, and 20 mg/ml, respectively.
Is rifampin water soluble?
Solubility : Soluble in water (pH 4.3) (1.3 mg/ml), chloroform, ethyl acetate, methanol (25 mg/ml), DMSO (25 mg/ml), dimethyl sulfoxide, tetrahydrofuran, and water (pH 7.3) (2.5 mg/ml at 25° C).
Is DMSO a better solvent than ethanol?
Ethanol and dimethyl sulfoxide (DMSO) are commonly used solvents employed as vehicles for hydrophobic compounds. Since DMSO was better tolerated than ethanol, it is thus recommended to prefer DMSO over ethanol in the case of a similar solubility of a given compound.
How do you make ciprofloxacin stock solution?
Ciprofloxacin reagent preparation
- Dissolve 25 mg of ciprofloxacin powder into 25 mL of DEPC water to make 1 mg/mL ciprofloxacin stock solution.
- Dilute the stock solution by adding 80 µL of that solution to 8 mL of DEPC water with 0.1% Tween 20, resulting in a 10 µg/mL concentration.
How do you get 50mg in mL?
To prepare 50 mg/mL BCIP disodium salt, dissolve 0.5 g in 10 mL H2O. To prepare 50 mg/mL BCIP p-toluidine salt, dissolve 0.5 g in 10 mL DMF. Store BCIP stock solutions at 4°C.
How do you dissolve rifampicin in DMSO?
Alternatively, you can dissolve the rifampin in DMSO at 100 mg/mL or methanol at 25 mg/mL and then dilute in PBS depending on your required dose.
How do you dilute rifampicin?
Reconstitute the lyophilized powder by transferring 10 mL of sterile water for injection to a vial containing 600 mg of rifampin for injection. Swirl vial gently to completely dissolve the antibiotic. The reconstituted solution contains 60 mg rifampin per mL and is stable at room temperature for up to 30 hours.
Can you mix alcohol and DMSO?
DMSO does not cause an accelerated absorption of alcohol but leads to a faster decline of the blood alcohol level.
Can DMSO be mixed with ethanol?
Both of them are commonly used in microwave heating as polar solvents. Several works have demonstrated that DMSO-ethanol mixture exhibits properties deviating from ideal due to the intrinsical variation of hydrogen bonding interaction55,56,57,58.
How do you make erythromycin stock?
For a stock solution of 10 mg/mL:
- 200 mg Erythromycin.
- 20 mL 100% ethanol (in fume hood)
- Do not filter sterilize—the ethanol may dissolve the filter.
How do you make a rifampicin stock solution?
50 mg/ml Rifampicin Stock Solution 1. Weigh 0.5 g of rifampicin [Rifampicin, GoldBio Catalog # R-120, (CAS 13292-46-1, MW = 822.94 g/mol)]. 2. Add 10 ml of 100% methanol. Dissolve completely. 3. Stock may be kept at -20°C for 1 year. Rifampicin may precipitate in the freezer. Dissolve again before use. GOLDBIO, GOLD BIOTECHNOLOGY’ discover more.
What happens when you add methanol to rifampin?
If i add more methanol so it will change the concentration of rifampin from 150 mg/ml to something else kindly suggest for this to reuse the same antibiotic with 150 mg/ml concentration. How to dissolve Rifampicin in saline solution?
What is rifampicin | CAS 13292-46-1?
Rifampicin | CAS 13292-46-1 is rated 5.0 out of 5 by 1 . Alternate Names: Rifampicin is also known as Rifampin. Application: Rifampicin is a rifamycin antibiotic that potently inhibits RNA polymerase, blocks RNA synthesis, and is an activator of PXR.
What is rifampicin used for?
Application: Rifampicin is a rifamycin antibiotic that potently inhibits RNA polymerase, blocks RNA synthesis, and is an activator of PXR. For Research Use Only. Not Intended for Diagnostic or Therapeutic Use. * Refer to Certificate of Analysis for lot specific data (including water content).